539508

PP2A₁, Bovine Kidney

• Synonyme(s) : Protein Phosphatase 2A₁
• Numéro de classification (Commission des enzymes): 3.1.3.16 (BRENDA, IUBMB)
• Code UNSPSC : 12352202

Propriétés

• Essai: ≥90% (SDS-PAGE)
• Niveau de qualité: 100
• Forme: liquid
• Activité spécifique: ≥750 units/mg protein
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Conditions d'expédition: wet ice
• Température de stockage: −20°C

Description

Description générale

Native, PP2A₁ from bovine kidney. A divalent cation-independent protein serine/threonine phosphatase involved in the control of numerous cellular processes. Has a catalytic subunit of 36 kDa, a subunit of 55 kDa, and a regulatory subunit of 60 kDa. Reported to associate with microtubules and to bind to purified tubulin polymerized by TAXOL^® Drug.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that will release 1.0 nmol of [³²P]P_i from ³²P-labeled phosphorylase per min at 30°C, pH 7.0.

Forme physique

In 50 mM Tris-HCl, 14 mM β-ME, 1 mM benzamidine, 1 mM EDTA, 100 µM PMSF, 50% glycerol, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Autres remarques

Hiraga, A. and Tamura, S. 2000. Biochem. J.346, 433.
Ferrigno, P., et al. 1993. Mol. Biol. Cell. 4, 669.
Guo, H. and Damuni, Z. 1993. Proc. Natl. Acad. Sci. USA90, 2500.
Hendrix, P., et al. 1993. J. Biol. Chem.268, 15267.
Amick, G.D., et al. 1992. Biochem. J. 287, 1019.
Cohen, P., et al. 1990. Trends Biochem. Sci.15, 98.
Damuni, Z., et al. 1989. J. Biol. Chem.264, 6412.1

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Taxol is a registered trademark of Bristol-Myers Squibb Co.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable