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527460

Sigma-Aldrich

PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb

liquid, Calbiochem®

Synonyme(s) :

Anti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Type de produit anticorps

primary antibodies

Clone

polyclonal

Forme

liquid

Contient

≤0.1% sodium azide as preservative

Espèces réactives

human, mouse

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
avoid repeated freeze/thaw cycles

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−70°C

Modification post-traductionnelle de la cible

phosphorylation (pThr451)

Informations sur le gène

human ... EIF2AK2(5610)

Description générale

Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
This PhosphoDetect Anti-PKR (pThr⁴⁵¹) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of PKR (pThr⁴⁵¹).

Immunogène

a synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)

Application

Immunoblotting (1:1000)

Immunohistochemistry (see comments)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In Dulbecco′s PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Remarque sur l'analyse

Positive Control
IFNγ-stimulated HeLa cells.

Autres remarques

Cuddihy, A.R., et al.1999. Oncogene8, 2690.
Srivastava, S.P., et al. 1998. J. Biol. Chem.273, 2416.
Clemens, M.J. 1997. Int. J. Biochem. Cell Biol.29, 945.
When used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

María Angel García et al.
PloS one, 6(8), e23887-e23887 (2011-09-03)
The chemotherapeutic drug 5-FU is widely used in the treatment of a range of cancers, but resistance to the drug remains a major clinical problem. Since defects in the mediators of apoptosis may account for chemo-resistance, the identification of new
Sharmistha Sarkar et al.
Viruses, 16(7) (2024-07-27)
Negative-strand RNA viruses form cytoplasmic inclusion bodies (IBs) representing virus replication foci through phase separation or biomolecular condensation of viral and cellular proteins, as a hallmark of their infection. Alternatively, mammalian cells form stalled mRNA containing antiviral stress granules (SGs)
Nishi R Sharma et al.
PLoS pathogens, 13(10), e1006677-e1006677 (2017-10-31)
TIA-1 positive stress granules (SG) represent the storage sites of stalled mRNAs and are often associated with the cellular antiviral response. In this report, we provide evidence that Kaposi's sarcoma-associated herpesvirus (KSHV) overcomes the host antiviral response by inhibition of

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