506114

p34^cdk1/Cyclin B, Human, Recombinant, S. frugiperda

• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.77

Propriétés

• Forme: liquid
• Niveau de qualité: 100
• Activité spécifique: ≥20,000 U/mL
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Activité étrangère: Protease, phosphatase, none detected
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr¹⁶¹ to form an active complex. MW: 34000(p34^Cdk1) and 55000(cyclinB).

Recombinant, human p34^Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34^cdc2 is inactive and requires association with cyclin B to form an active complex.

Avertissement

Toxicity: Standard Handling (A)

Définition de l'unité

One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.

Forme physique

In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ^® 35 Detergent, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.

Informations légales

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable