382159-M

PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb

liquid, Calbiochem^®

• Code UNSPSC : 12352203

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Contient: ≤0.1% sodium azide as preservative
• Espèces réactives: Xenopus, Tetrahymena sp., human, Drosophila, Caenorhabditis elegans
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Isotype: IgG
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pSer10)
• Informations sur le gène: human ... H3C1(8350)

Description

Description générale

Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

Recognizes the ~17 kDa histone H3 protein phosphorylated at Ser¹⁰.

This PhosphoDetect Anti-Histone H3 (pSer¹⁰) (7-20) Rabbit pAb is validated for use in Immunoblotting, ICC, IHC, IP for the detection of Histone H3 (pSer¹⁰) (7-20).

Immunogène

Human

a synthetic phosphopeptide [ARKpSTGGKAPRKQL(C)] corresponding to amino acids 7-20 surrounding the Ser¹⁰ phosphorylation site of human histone H3, conjugated to KLH

Application

Immunoblotting (0.5 µg/ml)

Immunocytochemistry (5 µg/ml)

Immunohistochemistry (see comments)

Immunoprecipitation (4 µg/10 µg acid-extracted proteins from colcemid-arrested HeLa cells)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Remarque sur l'analyse

Positive Control
Acid-extracted proteins from colcemid-arrested HeLa cells

Autres remarques

Ajiro, K., et al. 1996. J. Biol. Chem.271, 13197.
Chadee, D.N., et al. 1995. J. Biol. Chem.270, 20098.
Mahadevan, L.C., et al. 1991. Cell65, 775.

For immunocytochemistry, colcemid-arrested HeLa cells were fixed with 1% paraformaldehyde. Also detects histone H3 in Tetrahymena micronuclei. The antibody has been reported to detect mitotic cells in Xenopus, Caenorhabditis elegans, and Drosophila embryos. Does not cross-react with unphosphorylated histone H3. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal working dilution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable