17-300
Ral Activation Assay Kit
Non-radioactive Ral Activation Assay Kit can be used to precipitate Ral-A-GTP from cell lysates & detection by a Ral-A specific antibody.
Synonyme(s) :
Activation Assay Kit, Ral Assay Kit
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About This Item
Code UNSPSC :
12161503
eCl@ss :
32161000
Nomenclature NACRES :
NA.84
Produits recommandés
Niveau de qualité
Fabricant/nom de marque
Upstate®
Technique(s)
activity assay: suitable
affinity binding assay: suitable
Numéro d'accès NCBI
Conditions d'expédition
dry ice
Informations sur le gène
human ... RALA(5898)
Conditionnement
Kit capacity: 30 assays
Composants
Ral Activation Assay Buffer, 5X (Cat.# 20-196)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-Ral-A
Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Anti-Ral-A
Ral Assay Reagent (Ral BP1, agarose) (Cat.# 14-415)
Qualité
Routinely evaluated by affinity precipitation/immunoblot analysis. Ral-A-GTP was precipitated from EGF-stimulated Cos-7 cell lysates. The precipitated Ral-A-GTP was detected by Western immunoblot analysis using anti-Ral A.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Aquatic Chronic 3 - Eye Dam. 1
Code de la classe de stockage
10 - Combustible liquids
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Elena Tchevkina et al.
Oncogene, 24(3), 329-335 (2004-10-07)
RAS oncogenes play a critical role in oncogenic transformation and metastases formation. Here we show that Ha-ras greatly stimulates spontaneous metastatic activity of transformed cells through the Ras/RalGDS/RalA intracellular signaling pathway. Introduction of RalA alone leads to a drastic increase
2'-Methylseleno-modified oligoribonucleotides for X-ray crystallography synthesized by the ACE RNA solid-phase approach.
Barbara Puffer,Holger Moroder,Michaela Aigner,Ronald Micura
Nucleic Acids Research null
M C Araujo et al.
Biochemistry, 39(29), 8519-8525 (2000-07-29)
Quenched fluorescence peptides were used to investigate the substrate specificity requirements for recombinant wild-type angiotensin I-converting enzyme (ACE) and two full-length mutants bearing a single functional active site (N- or C-domain). We assayed two series of bradykinin-related peptides flanked by
Nicolas Personnic et al.
The FEBS journal, 281(13), 2977-2989 (2014-05-13)
Ral proteins are small GTPases that play critical roles in normal physiology and in oncogenesis. There is little information on the GTPase-activating proteins (GAPs) that downregulate their activity. Here, we provide evidence that the noncatalytic β subunit of RalGAPα1/2 β
Farnaz Yamoutpour et al.
Molecular cancer therapeutics, 7(11), 3586-3597 (2008-11-13)
Epidermal growth factor receptor variant III (EGFRvIII) is a constitutively active mutant form of EGFR that is expressed in 40% to 50% of gliomas and several other malignancies. Here, we describe the therapeutic effects of silencing EGFRvIII on glioma cell
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