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07-641

Sigma-Aldrich

Anti-MEK1 Antibody

Upstate®, from rabbit

Synonyme(s) :

Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1

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About This Item

Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41

Source biologique

rabbit

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

polyclonal

Espèces réactives

mouse, rat, human

Fabricant/nom de marque

Upstate®

Technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

Isotype

IgG

Numéro d'accès NCBI

NM_002755

Numéro d'accès UniProt

Q02750

Conditions d'expédition

dry ice

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MAP2K1(5604)

Spécificité

MEK1
Predicted to cross-react with rabbit based on sequence homology.

Immunogène

Peptide corresponding to amino acids 2-18 (PKKKPTPIQLNPAPDGS) of human MEK1.

Application

Research Category
Signaling
Research Sub Category
MAP Kinases
This Anti-MEK1 Antibody is validated for use in IP, WB for the detection of MEK1.

Qualité

Routinely evaluated by immunoblot on RIPA lysates from A431 and 3T3/A31 cells.

Description de la cible

45kDa

Liaison

Replaces: 04-376

Forme physique

70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol.
Format: Purified
Protein A purified

Stockage et stabilité

2 years at -20°C

Remarque sur l'analyse

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for mingels.

Informations légales

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Yasuyo Urasaki et al.
Nutrients, 12(2) (2020-02-06)
This study used nanofluidic protein posttranslational modification (PTM) profiling to measure the effects of six cannabidiol (CBD) oils and isolated CBD on the signaling pathways of a cultured SH-SY5Y neuronal cell line. Chemical composition analysis revealed that all CBD oils
Bacillus anthracis lethal toxin represses MMTV promoter activity through transcription factors.
Kang, Z; Webster Marketon, JI; Johnson, A; Sternberg, EM
Journal of Molecular Biology null
C/EBPbeta-2 confers EGF-independent growth and disrupts the normal acinar architecture of human mammary epithelial cells.
Bundy, L; Wells, S; Sealy, L
Molecular Cancer null
Brian J Girard et al.
The Journal of biological chemistry, 292(1), 339-350 (2016-11-25)
Cytoplasmic localization of proline, glutamic acid, leucine-rich protein 1 (PELP1) is observed in ∼40% of women with invasive breast cancer. In mouse models, PELP1 overexpression in the mammary gland leads to premalignant lesions and eventually mammary tumors. In preliminary clinical
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
Teague, M A, et al.
Proceedings of the National Academy of Sciences of the USA, 83, 7371-7375 (1986)
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