05-488-I
Anti-Pyk2/CAKβ Antibody
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41
Source biologique
mouse
Niveau de qualité
Conjugué
unconjugated
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
78, monoclonal
Poids mol.
calculated mol wt 115.78 kDa
observed mol wt ~110 kDa
Espèces réactives
human, rat
Conditionnement
antibody small pack of 100 μg
Technique(s)
immunocytochemistry: suitable
western blot: suitable
Isotype
IgG1
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Température de stockage
2-8°C
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
rat ... Ptk2b(50646)
Description générale
Protein-tyrosine kinase 2β (UniProt: P70600; also known as EC:2.7.10.2, Calcium-dependent tyrosine kinase, CADTK, Calcium-regulated non-receptor proline-rich tyrosine kinase, Cell adhesion kinase beta, CAK-beta, CAKB, Focal adhesion kinase 2, FADK 2, Proline-rich tyrosine kinase 2, Pyk2) is encoded by the Ptk2b (also known as Pak2, Pyk2) gene (Gene ID: 50646) in rat. Pky2 is a non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. Its protein kinase domain is localized in amino acids 425-683. Pyk2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It is highly expressed in pulmonary vein endothelial cells, lung, and brain. It localizes to the cytoplasmic side of the membrane. Pyk2 is activated by phosphorylation of tyrosine residues in response to various stimuli that elevate intracellular calcium levels. Tyr402 is the major autophosphorylation site, but it can also be phosphorylated by other kinases. This phosphorylation promotes interaction with Src and Src family members, leading to phosphorylations at Tyr579, Tyr580, and Tyr881. Phosphorylation at Tyr402, Tyr579 and Tyr580 is required for its optimal kinase activity and phosphorylation at Tyr881 is essential for its interaction with Grb2.
Spécificité
Clone 74 is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2β (Pyk2). It targets an epitope within the N-terminal half.
Immunogène
GST-tagged recombinant fragment corresponding to 412 amino acids from the N-terminal half of rat Protein-tyrosine kinase 2-beta (Pyk2).
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: A 1:250 dilution of this antibody detected Pyk2/CAKβ in Raji cell lysate.
Tested Applications
Western Blotting Analysis: A 1:250 dilution from a representative lot detected Pyk2/CAKβ in rat brain tissue lysate.
Western Blotting Analysis: A representative lot detected Pyk2/CAKβ in Western Blotting applications (Bernard-Trifilo, J.A., et. al. (2005). J Neurochem. 93(4):834-49).
Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Pyk2/CAKβ in E18 rat cortex neurons.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Pyk2/CAKβ, clone 74, Cat. No. 05-488-I, is a mouse monoclonal antibody that detects Protein-tyrosine kinase 2b and is tested for use in Immunocytochemistry and Western Blotting.
Forme physique
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at +2°C to +8°C from date of receipt.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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