04-746
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
culture supernatant, clone JY325, Upstate®
Synonyme(s) :
H3T3P, Histone H3 (phospho T3)
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
culture supernatant
Type de produit anticorps
primary antibodies
Clone
JY325, monoclonal
Espèces réactives
human
Fabricant/nom de marque
Upstate®
Technique(s)
dot blot: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
phosphorylation (pThr37)
Informations sur le gène
human ... H3C1(8350)
Description générale
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Spécificité
Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Immunogène
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325 (Rabbit Monoclonal Antibody) validated in WB, DB, Mplex, PIA to detect phospho-Histone H3 (Thr3) also known as H3T3P, Histone H3 (phospho T3).
WB, ChIP, BD, PIA
Qualité
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid, but not unmodified recombinant histone H3
Description de la cible
17 kDa
Forme physique
Cultured supernantant in 0.05% sodium azide.
Stockage et stabilité
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Svetlana M Medvedeva et al.
Molecules (Basel, Switzerland), 27(13) (2022-07-10)
This study represents the design and synthesis of a new set of hybrid and chimeric derivatives of 4,5-dihydro-4,4-dimethyl-1H-[1,2]dithiolo[3,4-c]quinoline-1-thiones, the structure of which the tricyclic fragment linearly bound or/and condensed with another heterocyclic fragment. Using the PASS Online software, among the
D Huertas et al.
Oncogene, 31(11), 1408-1418 (2011-08-02)
The approval of histone deacetylase inhibitors for treatment of lymphoma subtypes has positioned histone modifications as potential targets for the development of new classes of anticancer drugs. Histones also undergo phosphorylation events, and Haspin is a protein kinase the only
Ujué Fresán et al.
PLoS genetics, 16(8), e1008962-e1008962 (2020-08-05)
Haspin, a highly conserved kinase in eukaryotes, has been shown to be responsible for phosphorylation of histone H3 at threonine 3 (H3T3ph) during mitosis, in mammals and yeast. Here we report that haspin is the kinase that phosphorylates H3T3 in
Peiling Wang et al.
Journal of cellular physiology, 235(5), 4508-4519 (2019-10-19)
Haspin (Haploid Germ Cell-Specific Nuclear Protein Kinase) is a serine/threonine kinase pertinent to normal mitosis progression and mitotic phosphorylation of histone H3 at threonine 3 in mammalian cells. Different classes of small molecule inhibitors of haspin have been developed and
Jing Wang et al.
The Plant journal : for cell and molecular biology, 91(2), 199-207 (2017-04-04)
1RS.1BL translocations are centric translocations formed by misdivision and have been used extensively in wheat breeding. However, the role that the centromere plays in the formation of 1RS.1BL translocations is still unclear. Fluorescence in situ hybridization (FISH) was applied to detect
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