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803618

Sigma-Aldrich

DTME (dithio-bis-maleimidoethane)

Synonyme(s) :

1,1′-(dithiodi-2,1-ethanediyl)bis-1H-Pyrrole-2,5-dione, 1-[2-[[2-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]dithio]ethyl]-1H-pyrrole-2,5-dione, DTME

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About This Item

Formule empirique (notation de Hill) :
C12H12N2S2O4
Numéro CAS:
71865-37-7
Poids moléculaire :
312.36
Numéro MDL:
MFCD01863385
Code UNSPSC :
12352200
ID de substance PubChem :
329768821
Nomenclature NACRES :
NA.22

Essai

≥90%

Forme

powder

Poids mol.

312.37

Pertinence de la réaction

reagent type: cross-linking reagent

Conditions de stockage

desiccated

Solubilité

DMSO or DMF: soluble

Conditions d'expédition

ambient

Température de stockage

2-8°C

Chaîne SMILES 

O=C(C=CC1=O)N1CCSSCCN2C(C=CC2=O)=O

InChI

1S/C12H12N2O4S2/c15-9-1-2-10(16)13(9)5-7-19-20-8-6-14-11(17)3-4-12(14)18/h1-4H,5-8H2

Clé InChI

SGVWDRVQIYUSRA-UHFFFAOYSA-N

Description générale

DTME is a homobifunctional, maleimide crosslinker for conjugation between sulfhydryl groups (-SH). Such bismaleimide crosslinkers are commonly used to explore and characterize protein structure (i.e., oligomerization) or protein interactions. DTME is similar in length to BMH but differs in containing a disulfide bond in its spacer arm, allowing crosslinks with DTME to be cleaved with reducing agent such as dithiothreitol (DTT).

Caractéristiques et avantages

  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
  • Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent

Attention

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Classification des risques

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

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