During Quality Control, no testing to performed involving confocal microscopy. Conjugate 5-aminofluorescein (AFL)-human serum albumin has been used to stain brain cells, although it does not appear confocal microscopy was performed. Below is the reference: https://www.sigmaaldrich.com/tech-docs/paper/250406
201626
Fluoresceinamine, isomer I
Synonyme(s) :
5-Aminofluorescein
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250 MG
85,10 $
1 G
240,00 $
5 G
979,00 $
About This Item
Formule empirique (notation de Hill) :
C20H13NO5
Numéro CAS:
Poids moléculaire :
347.32
Beilstein:
48395
Numéro CE :
Numéro MDL:
Code UNSPSC :
12171500
ID de substance PubChem :
Nomenclature NACRES :
NA.47
85,10 $
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Produits recommandés
Forme
powder
Niveau de qualité
Pf
223 °C (dec.) (lit.)
Solubilité
methanol: 5 mg/mL
λmax
496 nm
Application(s)
diagnostic assay manufacturing
hematology
histology
Température de stockage
room temp
Chaîne SMILES
Nc1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI
1S/C20H13NO5/c21-10-1-4-14-13(7-10)19(24)26-20(14)15-5-2-11(22)8-17(15)25-18-9-12(23)3-6-16(18)20/h1-9,22-23H,21H2
Clé InChI
GZAJOEGTZDUSKS-UHFFFAOYSA-N
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Catégories apparentées
Description générale
Fluoresceinamine, isomer I belongs to the class of derivatized fluoresceins.
Application
Fluoresceinamine, isomer I is suitable for use in a specific and sensitive spectrophotometric method for determining nitrite. It has been used to fluorescently tag nanoparticles through a competitive carboxyl-amine coupling reaction to visualize nanoparticle internalization.
Mention d'avertissement
Warning
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
P Gribbon et al.
Biophysical journal, 77(4), 2210-2216 (1999-10-08)
Hyaluronan (HA) is a highly hydrated polyanion, which is a network-forming and space-filling component in the extracellular matrix of animal tissues. Confocal fluorescence recovery after photobleaching (confocal-FRAP) was used to investigate intramolecular hydrogen bonding and electrostatic interactions in hyaluronan solutions.
Surface modification of cell culture carriers: routes to anhydride functionalization of polystyrene.
Mirko Nitschke et al.
Colloids and surfaces. B, Biointerfaces, 90, 41-47 (2011-10-22)
Physico-chemical and topographical cues allow to control the behavior of adherent cells. Towards this goal, commercially available cell culture carriers can be finished with a laterally microstructured biomolecular functionalization. As shown in a previous study [Biomacromolecules 4 (2003) 1072], the
D M Kranz et al.
The Journal of biological chemistry, 257(12), 6987-6995 (1982-06-25)
Binding of fluorescyl ligand by five IgG anti-fluorescyl hybridoma proteins (4-4-20, 6-10-6, 20-4-4, 20-19-=1, 20-20-3) was examined. Relative reduction in fluorescence of bound fluorescein, deuterium oxide (D2O)-induced enhancement of fluorescence, and the effects of pH on binding kinetics were measured
pH sensor based on immobilized fluoresceinamine
Saari L A
Analytical Chemistry, 54, 821-823 (1982)
S Miyauchi et al.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 12(1), 27-34 (1996-01-01)
The protective efficacy of sodium hyaluronate (Na-HA) on the corneal endothelium against the damage induced by sonication was investigated using enucleated rabbit eyes and Na-HA fluorescence labeled with 5-aminofluorescein (FA-HA). The anterior chamber was reformed by injecting a 1% solution
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Can this be used to stain cells and visualize them under the confocal microscope?
1 answer-
Helpful?
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Dear manager, I want to ask you 2 questions about this product. 1. Is it a flurescence dye? Can you show me its adsorption or emission spectrum? 2. Is the amine group reactive?
1 answer-
Yes, Fluoresceinamine, isomer I is classified as belonging to a group of derivatized fluorosceins. The product is fluorescent. Although no quality control testing is performed to check for excitation and emission, the dye is expected to excite around 490 nm and emit around 520 nm.
As the H2N- group is not acetylated or protonated, the amine group can be considered reactive. There is literature available stating Fluoresceinamine, isomer I can be covalently bound to human serum albumin.
Helpful?
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What is the solubility of Fluoresceinamine, isomer I, CAS 3326-34-9?
1 answer-
Soluble in methanol at a concentration of 5 mg/mL. Soluble in DMSO at a concentration of ≥ 32 mg/mL.
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