Biotinylated Peptides

SigmaScreen™ HC Streptavidin Coated Plates, (P9740)

Product No: S6940 (96-well, clear)
Product No: S8815 (384-well, clear)

Our Streptavidin HC (High-Capacity) multiwell plates are prepared with a highly-porous, high-density streptavidin coating. Streptavidin has similar biotin-binding characteristics as avidin. Streptavidin can bind 4 moles of biotin per mole of protein with high selectivity and affinity (K_d~10^-15). Unlike avidin, streptavidin has a near neutral pI that alleviates non-specific binding commonly associated with the basic avidin protein.

These plates provide high capacity affinity binding of biotinylated compounds
making them useful for:

• Purification of biotinylated compounds from heterogeneous mixtures.
• Preparation of high capacity affinity matrices for purification of binding partners to immobilized biotinylated compounds.
• Recovery of binding partners and/or biotin compounds for subsequent analysis, e.g. mass spectrometry (MS) and SDS-PAGE.
• Recovery of ICAT™ labeled compounds.

Biotin Binding Capacity

Product Number		Minimum pmoles Biotin Binding Capacity per Well
S 6940	96-well	300
S 8815	384-well	150

Capacity for biotin based on binding biotin-fluorescein. Steric hindrance will reduce the capacity of the plate when binding larger (> 5 kDa) molecules.
 

View Protocol Diagram

Data

• Selectivity of Binding on Streptavidin HC
• Comparison of Streptavidin HC Plate to Monomeric Avidin Agarose