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form
suspension
shelf life
2 yr at −20‑0 °C
pH
~5
capacity
≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)
storage temp.
−20°C
General description
The PHOS-Select™ iron affinity gel is a novel iron [Fe(III)] chelate matrix. This matrix provides high capacity affinity binding of molecules containing phosphate groups.
Application
Phos-Select™ products facilitate a number of applications including:
- affinity enrichment of phosphopeptides obtained from protein tryptic digests
- direct transfer of phosphocompounds for analysis (HPLC or mass spectrometry)
- enrichment of phosphopeptides by immobilized metal affinity chromatography (IMAC)
Other Notes
Phos-Select™ Iron Affinity Gel is a novel iron [Fe(III)] chelate matrix based on Sigma′s NTA analog chelate ligand. The matrix provides high capacity affinity binding of molecules containing phosphate groups.
Physical form
aqueous glycerol suspension
Legal Information
PHOS-Select is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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To better understand the acute inflammatory mechanisms, the modulation, and to investigate the key node in predicting inflammatory diseases, high-sensitivity LC-MS/MS-based proteomics and phosphoproteomics approaches were used to identify differential proteins in RAW264.7 macrophages with lipopolysaccharide (LPS). Furthermore, differential proteins
Jie Gao et al.
Scientific reports, 7, 40852-40852 (2017-01-21)
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Protein modifications are crucial for disease study. Analysis methods are key.
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