Skip to Content
MilliporeSigma
HomeProductsProtein BiologyProteins & EnzymesTissue DissociationCollagenase

Collagenase

collagenase bottle

Collagenase is an endopeptidase that effectively breaks down the collagen at its peptide bond regions under physiological pH & temperature conditions. Being a protein, it is highly sensitive to pH, temperature, and several other factors that can lead to protein denaturation. The activity, conformation, and properties of collagenase are highly influenced by external factors.

Collagenase consists of different types, such as I, II, III, IV, V, VI VII, VIII, XI, and a special collagenase for liver cells. Collagenase from Clostridium histolyticum is classified into 6 groups, namely, viz, alpha, beta, gamma, delta, epsilon, and zeta. It serves as an important catalyst and finds applications in stem cell biology, cancer research, and immunology.

Collagenase for cell/tissue isolation

Collagenase supports a wide range of research as it can be used to isolate cells/tissues by degrading collagen in the skin, blood vessels, tendons, and bones. The type of collagenase is chosen based on the specific type of cell or tissue that needs to be isolated. Collagenase type I is suitable for the isolation of fat, lung, epithelial, and adrenal tissue cells. Collagenase type II is ideal for the separation of the liver, thyroid, bone, heart, and salivary gland tissues. Collagenase type IV can break down a variety of tissues, and type V is applicable in the isolation of pancreatic islet tissues by separating the connective tissues into single cells. Collagenase disaggregation is ideal for culturing mouse kidneys, human adult, and fetal brains.

Collagenase for cell culture

Collagenase is gentle, requires no agitation, and dissociates well at optimum temperature and pH. Therefore, it is ideal for culturing mouse kidneys, human adult and fetal brains, human tumors, and epithelial tissues.

Collagenase as a therapeutic

Collagenase plays an important role in various clinical applications, including the treatment of lumbar disc and sciatic nerve issues, trauma therapy, rheumatoid arthritis, and osteoarthritis.

Related Resources
Tissue Dissociation Guide: Collagenase, Dispase, and Liberase Enzyme Types
Collagenase Guide.Collagenases, enzymes that break down the native collagen that holds animal tissues together, are made by a variety of microorganisms and by many different animal cells.
Core Cell Culture
Creating conditions similar to those that exist in vivo is essential for cell culture. To ensure a consistent environment throughout your experiment it requires well-characterized reagents and high-quality products. From sterile media to application-tested supplements and a growing animal component-free product range, we have the tools to help you optimize cell growth.
Related Product Categories
Roche® Life Science Products
Roche Life Science Products provide dependable reagents for breakthrough research, ensuring high-quality, reproducible results.
Cell Culture Troubleshooting
Expert tips for correcting common problems with cell culture including bacterial and fungal contamination, debris in culture media, cell detachment or clumping, slow growth, and low viability.
Tissue Dissociation
Discover efficient cell detachment: reliable enzymes - trypsin, collagenase, papain, nucleases, hyaluronidase, elastase, protease XIV.
Cell Dissociation Reagents
Enzymes like trypsin and collagenase are essential for the detachment of adherent cells and solid tissues for subculture and experiments. Recombinant trypsin, solutions containing EDTA, and non-enzymatic solutions provide stabler, gentler options for wider culture applications.
Related Applications
Cell Culture & Cell Culture Analysis
Methods for bacterial, plant, and mammalian cell culture, transfection, gene editing, assays, imaging, and troubleshooting.
Sign In To Continue

To continue reading please sign in or create an account.

Don't Have An Account?