G005559
HPLC Analysis of PAHs on SUPELCOSIL™ LC-PAH
application for HPLC
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About This Item
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technique(s)
HPLC: suitable
test parameters
column: SUPELCOSIL LC-PAH, 15 cm x 4.6 mm I.D., 5 μm particles (58318)
mobile phase: [A] water, [B] methanol
gradient: 55 to 100% B in 5 min; held at 100%B for 10 min
flow rate: 1.5 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 5 μL
sample: EPA 610 mix
suitability
application for HPLC
application(s)
environmental
Analysis Note
PAHs are traditionally evaluated using an acetonitrile/water gradient. Recently customers are looking for alternatives to this method using a methanol/water gradient due to the risk of an another acetonitrile shortage, solvent costs, and toxicity to the environment. This analysis demostrates the separation using a methanol gradient.
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Analyte
Description
Naphthalene
Acenaphthene
Fluorene
Phenanthrene
Anthracene
Fluoranthene
Pyrene
Benz[a]anthracene solution
Chrysene
Benzo[b]fluoranthene
Benzo[k]fluoranthene
Benzo[a]pyrene
Benzo[ghi]perylene
Indeno[1,2,3-cd]pyrene
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analytical column
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