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G004511

HPLC Analysis of Pyroglutamic Acid Enantiomers on Astec® CHIROBIOTIC® T

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)
mobile phase: [A] acetonitrile; [B] methanol; [C] acetic acid; [D] triethylamine; (54.5:45:0.3:0.2, A:B:C:D)
flow rate: 1 mL/min
column temp.: 23 °C
detector: UV, 220 nm
injection: 5 μL
sample: 1 mg/mL in methanol

suitability

application for HPLC

application(s)

life science and biopharma

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analyte

Description
    DL-Pyroglutamic acid, puriss., ≥99.0% (T)

analytical column

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