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Key Documents

G003699

HPLC Analysis of Propoxyphene and Methadone on Ascentis® Phenyl

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)
mobile phase: [A] water with 0.1% ammonium acetate: [B] methanol; (20:80, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 220 nm
injection: 10 μL
sample: 50 μg/mL in 20:80, water:methanol

suitability

application for HPLC

application(s)

forensics and toxicology

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

analytical column


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