ZRB2815

Anti-PABP-1 Antibody, clone 1D5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): PAB1, PABP, PABP-1, PABP1, PABPC2, Poly(A)-binding protein 1
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 70.67 kDa; observed mol wt ~70 kDa
• purified by: using Protein A
• species reactivity: human, mouse
• species reactivity (predicted by homology): monkey, bovine, rat, horse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgGκ
• epitope sequence: C-terminal
• Protein ID accession no.: NP_002559
• UniProt accession no.: P11940
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... PABPC1(26986)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D5 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects PABP-1. It targets an epitope within 21 amino acids from the C-terminal part.

Immunogen

KLH-conjugated linear peptide corresponding to 21 amino acids from the C-terminal region of human PABP-1.

Application

Quality Control Testing

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PABP-1 in A549 cell lysate.

Tested Applications

Western Blotting Analysis: A representative lot of this antibody detected PABP-1 in A549, NIH/3T3 cell lysates (1:1,000 dilution), and human testis lysate (1:10,000 dilution).

Flow Cytometry Analysis: 1 µg from a representative lot detected PABP-1 in one million HEK-293 cells .
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected PABP-1 in human testis tissue sections.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected PABP-1 in HEK-293 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Polyadenylate-binding protein 1 (UniProt: P11940; also known as PABP-1; Poly(A)-binding protein 1) is encoded by the PABPC1 (also known as PAB1;PABP;PABP1, PABPC2) gene (Gene ID: 26986) in human. Polyadenylate-binding protein 1 (PABP1) is a multifunctional protein characterized by four RNA recognition motifs (RRMs) that bind to the poly(A) tail of mRNA molecules. Additionally, PABP1 has a proline-rich C-terminal domain involved in protein-protein interactions, which is essential for its role in regulating mRNA metabolic processes. PABP1 plays a critical role in the regulation of mRNA stability and translation. By binding to the poly(A) tail of mRNAs, it enhances the stability of the mRNA and facilitates its efficient translation by ribosomes. PABP1 is also involved in the modulation of mRNA polyadenylation during transcription and influences mRNA nuclear export and deadenylation. PABP1 is ubiquitously expressed across various tissues, reflecting its fundamental role in mRNA metabolism. The expression levels of PABP1 can vary depending on cellular conditions and stress, indicating its involvement in adaptive cellular responses. Dysfunction or abnormal levels of PABP1 are associated with several human diseases. For instance, altered PABP1 expression is linked to cancer, where it can affect cell proliferation and tumor progression by modulating the stability and translation of mRNAs encoding oncogenes and tumor suppressors. Additionally, PABP1 has been implicated in viral infections; many viruses manipulate PABP1 to preferentially translate viral mRNAs. Neurodegenerative diseases such as Amyotrophic Lateral Sclerosis and Frontotemporal Lobar Degeneration have also been associated with mutations in the PABP1 gene, affecting its normal function.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable