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T7290

Sigma-Aldrich

Tyrphostin 25

≥98%

Synonym(s):

(3,4,5-Trihydroxybenzylidene)malononitrile

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About This Item

Empirical Formula (Hill Notation):
C10H6N2O3
CAS Number:
118409-58-8
Molecular Weight:
202.17
MDL number:
MFCD00133900
UNSPSC Code:
12352200
PubChem Substance ID:
24278753
Pricing and availability is not currently available.

Assay

≥98%

potency

3 μM IC50

solubility

DMSO: >50 mg/mL, clear, yellow to orange

storage temp.

2-8°C

SMILES string

Oc1cc(cc(O)c1O)\C=C(\C#N)C#N

Gene Information

human ... EGFR(1956)

Application

Tyrphostin 25 has been used for studying its effect on transcriptional repression induced by the adenoviral oncoprotein, E1a, in HeLa cells[1]. Tyrphostin 25 has also been used to study the migration inhibition in IEC-6 cells[2].

Biochem/physiol Actions

EGFR tyrosine kinase inhibitor.
Tyrphostin 25 is a small molecule inhibitor of epidermal growth factor (EGF) receptor kinase function. This molecule associates with the substrate subsite of the protein tyrosine kinase (PTK) domain[3],[4].

Preparation Note

Tyrphostin 25 is soluble in DMSO at a concentration that is greater than 50 mg/ml and yields a clear, yellow to orange solution.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
045H0179
082H0389
087K1109
025K0869
059H0566

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Israel Rubinstein
Journal of applied physiology (Bethesda, Md. : 1985), 103(1), 184-189 (2007-04-14)
The purpose of this study was to determine whether protein tyrosine kinase, a ubiquitous family of intracellular signaling enzymes that regulates endothelial cell function, modulates bradykinin- and substance P-induced increase in macromolecular efflux from the intact hamster cheek pouch microcirculation.
A R Shenoy et al.
Biochimie, 87(6), 557-563 (2005-05-24)
A number of genes similar to mammalian Class III nucleotide cyclases are found in mycobacteria, and biochemical characterization of some of these proteins has indicated that they code for adenylyl cyclases, with properties similar to the mammalian enzymes. Our earlier
Lian Hua Fang et al.
FEBS letters, 512(1-3), 282-286 (2002-02-20)
This study was undertaken to determine the role of tyrosine kinase on intracellular Ca(2+) ([Ca(2+)](i)), myosin light chain (MLC) phosphorylation, and contraction caused by norepinephrine (NE) in rat aorta. NE induced a sustained contraction with an increase of [Ca(2+)](i). On
Yu-Jing Gao et al.
Cardiovascular research, 71(2), 363-373 (2006-06-08)
Recent studies have demonstrated that perivascular adipose tissue (PVAT) releases vascular relaxation factor(s). In this study, we examined if PVAT releases other vasoactive factors in response to perivascular nerve activation by electrical field stimulation (EFS). In Wistar-Kyoto rats, rings of
Maria Grazia Zizzo et al.
European journal of pharmacology, 521(1-3), 133-138 (2005-09-28)
The aim of this study was to investigate the signal transduction mechanisms underlying the inhibitory effect induced by pituitary adenylate cyclase activating peptide (PACAP-27) on the spontaneous contractile activity of longitudinal muscle of mouse ileum. Mechanical activity of ileal segments
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