SRP6260

SERPINA1/A1AT human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Synonym(s): A1A, A1AT, AAT, MGC23330, MGC9222, PI, PI1, PRO2275, SPAAT, SerpinA1, alpha1AT
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in HEK 293 cells
• tag: 6-His tagged (C-terminus)
• Assay: ≥95% (SDS-PAGE)
• form: lyophilized powder
• potency: <4.5 nM IC₅₀
• mol wt: calculated mol wt 45.1 kDa; observed mol wt 55-60 kDa (DTT-reduced. Protein migrates due to glycosylation. Glu 25 is the predicted N-terminus.)
• packaging: pkg of 10 μg
• impurities: <1 EU/μg endotoxin (LAL test)
• UniProt accession no.: P01009
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... SERPINA1(5265)

Description

General description

Serpin A1 also known as α-1-antitrypsin (A1AT), serum trypsin inhibitor, α-1 proteinase inhibitor (A1PI), AAT, which belongs to the serpin family. Most serpins inactivate enzymes by binding to them covalently, requiring very high levels to perform their function. Like all serine protease inhibitors, A1AT has a characteristic secondary structure of β sheets and α helices. Serpin A1 / A1AT is inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form of SerpinA1 inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Serpin A1 / A1AT protects tissues from enzymes of inflammatory cells, especially neutrophil elastase. Defects in SERPINA1 are the cause of α-1-antitrypsin deficiency (A1ATD).

Physical form

Lyophilized from 0.22 μm filtered solution in PBS. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable