SRP0251

STK33 Active human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

• Synonym(s): Serine/threonine kinase 33
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in baculovirus infected insect cells
• Assay: ≥65% (SDS-PAGE)
• form: aqueous solution
• mol wt: 84 kDa
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• NCBI accession no.: NM_030906.2
• UniProt accession no.: Q9BYT3
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... STK33(65975)

Description

General description

STK33 (GenBank Accession No. NM_030906.2), amino acids 2-end, with N-terminal GST-tag, MW = 84 kDa, expressed in a Baculovirus-infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of MBP substrate per minute at room temperature

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.