SAB5702998

Anti-UPF1/RENT1 Antibody, clone 5D5C4, Rabbit Monoclonal

• Synonym(s): HUPF1, NORF1, RENT1, pNORF1, smg-2
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: rabbit
• Quality Level: 100
• material: colorless
• clone: 5D5C4, monoclonal
• form: liquid
• mol wt: 140 kDa
• species reactivity: rat, human, rat
• concentration: 0.4mg/ml
• technique(s): immunofluorescence: 1:50 - 1:200; immunoprecipitation (IP): 1:50 - 1:200; western blot: 1:500 - 1:2000
• color: colorless
• isotype: IgG
• immunogen sequence: MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLL
• UniProt accession no.: Q92900
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... UPF1(5976)

Description

General description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human UPF1/RENT1 (Q92900).

PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Application

WB,IF/ICC, IP

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1