SAB4702052

Monoclonal Anti-CD25 PE-Cyanine7 produced in mouse

• Synonym(s): IDDM10, IL-2-RA, IL-2R α chain, IL2-RA, TAC antigen, TCGFR, p55
• UNSPSC Code: 12352203

Properties

• conjugate: PE-Cy7 conjugate
• Quality Level: 100
• antibody product type: primary antibodies
• clone: MEM-181, monoclonal
• form: buffered aqueous solution
• species reactivity: human
• technique(s): flow cytometry: suitable
• isotype: IgG1
• UniProt accession no.: P01589
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IL2RA(3559)

Description

General description

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

Specificity

The antibody MEM-181 reacts with an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Immunogen

PHA-activated peripheral blood leucocytes

Physical form

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preparation Note

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Storage and Stability

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Other Notes

The antibody solution should be gently mixed before use.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable