SAB4200639

Monoclonal Anti-DHX9/RHA antibody produced in rat

clone 8E3, purified from hybridoma cell culture

• Synonym(s): Anti-ATP−dependent RNA helicase A, Anti-ATP-dependent RNA helicase A, Anti-DDX9, Anti-DEAD/H (Asp−Glu−Ala−Asp/His) box polypeptide 9, Anti-DEAH (Asp−Glu−Ala−His) box polypeptide 9, Anti-DEAH (Asp-Glu-Ala-His) box helicase 9, Anti-DEAH (Asp-Glu-Ala-His) box polypeptide 9, Anti-DEAH box protein 9, Anti-DHX9, Anti-Ddx9, Anti-HEL-5, Anti-LKP, Anti-NDH2, Anti-NDHII, Anti-Nuclear DNA helicase II (NDHII), Anti-leukophysin, Anti-mHEL-5, Anti-nuclear DNA helicase II, RHA, RNA helicase A
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 8E3, monoclonal
• form: buffered aqueous solution
• species reactivity: mouse, hamster, rat, bovine, canine, monkey, human
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 1-2 μg/mL using using whole extracts of HeLa cells.; immunofluorescence: 5-10 μg/mL using using HeLa cells
• isotype: IgG1
• UniProt accession no.: Q08211
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: Monkey ... DHX9(715453) ; canine ... DHX9(480032) ; human ... DHX9(1660) ; mouse ... Dhx9(13211) ; rat ... Dhx9(304859)

Description

General description

DHX9 (DEAH (Asp-Glu-Ala-His) box helicase 9) also known as RNA Helicase A, nuclear DNA helicase II (NDHII), belongs to the DExD/H family of helicase superfamily 2, that exhibits both RNA and DNA helicase activity in an ATP dependent reaction.

Immunogen

synthetic peptide corresponding to the N-terminal region of mouse DHX9

Application

Monoclonal Anti-DHX9/RHA antibody produced in rat has been used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

DHX9 (DEAH (Asp-Glu-Ala-His) box helicase 9) is involved in RNA metabolism including RNA secondary structure rearrangement, RNA-protein interactions, RNA turnover, ribosome biogenesis, translation and small RNA metabolism. It participates in the replication cycles of retroviruses and other RNA viruses. DHX9 is implicated in multiple steps of HIV-1 replication, including transcription, export of unspliced viral RNA from the nucleus, translation, particle assembly and reverse transcription.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable