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R6878

Sigma-Aldrich

Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse

~2 mg/mL, clone RB-10, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-phospho-Rb (pSer795)

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RB-10, monoclonal

form

buffered aqueous solution

mol wt

antigen 110 kDa

species reactivity

mouse, rat, human, bovine, hamster

concentration

~2 mg/mL

technique(s)

immunohistochemistry: suitable using 3% paraformaldehyde fixation, 0.15% Triton X-100 permeabilization
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using a whole cell extract of human melanoma cell line G-361

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pSer795)

Gene Information

human ... RB1(5925)
mouse ... Rb1(19645)
rat ... Rb1(24708)

General description

Monoclonal Anti-phospho-Retinoblastoma (Rb) (pS795) (mouse IgG1 isotype) is derived from the RB-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic phospho peptide corresponding to the C-terminal amino acids of human retinoblastoma protein, conjugated to KLH. Retinoblastoma proteins p107, p110 (also designated Rb, or pRb) and p130, referred to collectively as pocket proteins, constitute a nuclear protein family that share a common structural unit (the pocket) dedicated to binding certain transcription factors and thereby regulating cellular proliferation. Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase and proteins with conserved LXCXE motif.

Immunogen

synthetic phosphopeptide corresponding to the C-terminal amino acids 791-804 (pSer795) of human retinoblastoma protein conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-phospho-Retinoblastoma (pSer795) antibody produced in mouse has been used in:
  • immunoblotting
  • immunocytochemistry
  • immunofluorescence
  • fluorescence-activated cell sorting (FACS) assays
  • enzyme linked immunosorbent assay (ELISA)

Biochem/physiol Actions

Retinoblastoma (Rb) exerts its function by repressing the transcription of cellular genes required for DNA replication and cell division. It can be phosphorylated at multiplicity of sites in vitro by cell division cycle protein 2 (Cdc2), cyclin-dependent kinase (Cdk2) and Cdk4/Cdk6 kinase complexes. Phosphorylation of pS795 is required to inactivate Rb imposed growth suppression.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The E2F1-3 transcription factors are essential for cellular proliferation
Wu L, et al.
Nature, 414(6862), 457-457 (2001)
Aurora-A overexpression is linked to development of aggressive teratomas derived from human iPS cells
Ohmine S, et al.
Oncology Reports, 39(4), 1725-1730 (2018)
Karen J Meaburn et al.
Aging cell, 6(2), 139-153 (2007-02-06)
A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear
Cellular mechanisms of tumour suppression by the retinoblastoma gene
Burkhart DL and Sage J
Nature Reviews. Cancer, 8(9), 671-671 (2008)
Yan Li et al.
Molecular medicine reports, 14(3), 2222-2230 (2016-07-20)
Ginsenoside Rg3 (Rg3) is one of the primary constituents isolated from ginseng, and has been found to exhibit cytotoxic effects against cancer cells. The present study aimed to investigate the effects of Rg3 on human multiple myeloma cell proliferation and

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