P7130
Puromycin aminonucleoside
Synonym(s):
3′-Amino-3′-deoxy-N6,N6-dimethyladenosine
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All Photos(4)
About This Item
Empirical Formula (Hill Notation):
C12H18N6O3
CAS Number:
Molecular Weight:
294.31
Beilstein:
93902
EC Number:
MDL number:
UNSPSC Code:
51281912
PubChem Substance ID:
NACRES:
NA.85
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form
powder
Quality Level
solubility
H2O: soluble 50 mg/mL
antibiotic activity spectrum
Gram-positive bacteria
neoplastics
parasites
Mode of action
protein synthesis | interferes
storage temp.
2-8°C
SMILES string
CN(C)c1ncnc2n(cnc12)[C@@H]3O[C@H](CO)[C@H](N)[C@@H]3O
InChI
1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7+,9+,12-/m1/s1
InChI key
RYSMHWILUNYBFW-VENHTOENSA-N
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General description
Puromycin aminonucleoside is the aminonucleoside portion of the antibiotic puromycin. It is useful in nephrology research, like studies of focal and segmental glomerulosclerosis and in the induction of nephrosis in rats. The excretion of sodium and nitrite (NOx) metabolites in rats with puromycin aminonucleoside-induced nephrotic syndrome are studied. Puromycin aminonucleoside-induced nephrosis in rats has been studied with respect to the production of reactive oxygen species during the acute phase. Puromycin aminonucleoside is used to probe endothelial glycosaminoglycan synthesis in cultured glomerular endothelial cells and their relation to cell permeability.
Application
Puromycin aminonucleoside has been used:
- as a selection marker for the infected cells for the transfection of lentivirus
- as a component of fresh medium for transfection and infection assays
- to subcutaneously induce puromycin aminonucleoside nephrosis to investigate the mRNA and protein levels of nephrin and podocin before the onset of proteinuria
Biochem/physiol Actions
Puromycin aminonucleoside is used to study human glomerular disease by inducing damage of murine glomerular podocytes and is used to study glomerular function and morphology.
Other Notes
Keep container tightly closed in a dry and well-ventilated place.
Signal Word
Warning
Hazard Statements
Hazard Classifications
STOT RE 2
Target Organs
Kidney
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Ramzi Khalil et al.
The Journal of pathology, 247(2), 177-185 (2018-10-24)
Dynamin plays an essential role in maintaining the structure and function of the glomerular filtration barrier. Specifically, dynamin regulates the actin cytoskeleton and the turnover of nephrin in podocytes, and knocking down dynamin expression causes proteinuria. Moreover, promoting dynamin oligomerization
Ido Refaeli et al.
Scientific reports, 10(1), 9419-9419 (2020-06-12)
Dominant and recessive mutations in podocalyxin (PODXL) are associated with human kidney disease. Interestingly, some PODXL mutations manifest as anuria while others are associated with proteinuric kidney disease. PODXL heterozygosity is associated with adult-onset kidney disease and podocalyxin shedding into
Jeffrey A Kramer et al.
Environmental health perspectives, 112(4), 460-464 (2004-03-23)
Microarrays allow for the simultaneous measurement of changes in the levels of thousands of messenger RNAs within a single experiment. As such, the potential for the application of transcription profiling to preclinical safety assessment and mechanism-based risk assessment is profound.
Yuqiu Lu et al.
Kidney international, 92(5), 1119-1129 (2017-07-16)
Gene expression differs substantially among individual cells of the same type. We speculate that genes that are expressed in all but a portion of cells of a given cell type would be likely essential and required for either the cell
G N Marinides et al.
Kidney international, 37(2), 749-757 (1990-02-01)
The effects of dietary protein and converting enzyme inhibition (CEI) on chronic puromycin aminonucleoside nephropathy (PAN) were studied. PAN was induced with seven SQ injections of puromycin aminonucleoside 20 mg/kg over 10 weeks in male Sprague-Dawley rats. The rats were
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