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P2860

Sigma-Aldrich

Monoclonal Anti-PSF antibody produced in mouse

clone B92, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-Polypyrimidine tract-binding protein-associated splicing factor

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About This Item

MDL number:
MFCD03457573
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

B92, monoclonal

form

buffered aqueous solution

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
radioimmunoassay: suitable using cells
western blot: 2-4 μg/mL using whole extract of cultured HeLa cells

isotype

IgG1

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TAP1(6890)

General description

Monoclonal Anti-PSF (mouse IgG1 isotype) is derived from the B92 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a C57BL/6J mouse immunized with a lysate of mouse bone marrow-derived stromal cell line. Polypyrimidine tract-binding protein-associated splicing factor (PSF) is an ubiquitous nuclear matrix protein. It is characterized with two consensus RNA-binding domains and an unusual amino terminus rich in proline and glutamine residues.

Immunogen

lysate of mouse bone marrow-derived stromal cell line.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)
Monoclonal Anti-PSF antibody produced in mouse has been used in:
  • immunofluorescence
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

Polypyrimidine tract-binding protein-associated splicing factor (PSF) plays a pivotal role in mammalian spliceosome. PSF is also necessary for pre-mRNA splicing and transcription. The restricted expression of PSF suggests that it is involved in the control of neuronal-specific splicing events occurring at particular stages of neuronal differentiation and maturation. Monoclonal antibodies reacting specifically with PSF are useful tools for the molecular identification and characterization of the functional activity of PSF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Preparation Note

Purified from culture supernatant of hybridoma cells grown in a bioreactor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Paraspeckles are subpopulation-specific nuclear bodies that are not essential in mice
Nakagawa S, et al.
The Journal of cell biology, 193(1), 31-39 (2011)
Cytoplasmic Relocalization of TAR DNA-Binding Protein 43 Is Not Sufficient to Reproduce Cellular Pathologies Associated with ALS In vitro
Wobst HJ, et al.
Frontiers in Molecular Neuroscience, 10(7) (2017)
Wen Shen et al.
Nucleic acids research, 46(5), 2204-2217 (2018-02-02)
We reported previously that a 2' fluoro-modified (2' F) phosphorothioate (PS) antisense oligonucleotides (ASOs) with 5-10-5 gapmer configuration interacted with proteins from Drosophila behavior/human splicing (DBHS) family with higher affinity than PS-ASOs modified with 2'-O-(2-methoxyethyl) (2' MOE) or 2',4'-constrained 2'-O-ethyl
Differential nuclear localization and nuclear matrix association of the splicing factors PSF and PTB
Meissner M, et al.
Journal of Cellular Biochemistry, 76(4), 559-566 (2000)
Chungoo Park et al.
Scientific reports, 7(1), 11211-11211 (2017-09-13)
Rbfox RNA-binding proteins play important roles in the regulation of alternative pre-mRNA splicing, but their role in other gene regulatory mechanisms is not well understood. Here, we show that Rbfox2 is a novel constituent of cytoplasmic stress granules, the translational
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