MSP04
Membrane Scaffold Protein 1E1D1
recombinant, expressed in E. coli, buffered aqueous solution
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.26
Recommended Products
recombinant
expressed in E. coli
Assay
≥90% (SDS-GE)
form
buffered aqueous solution
shipped in
ambient
storage temp.
−20°C
General description
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E1D1 variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the acccompanying figure) of the original MSP1 sequence (which is known separately as MSP1D1), and also repeats the Helix 4 sequence of the original MSP1 sequence between the parent Helix 4 and Helix 5 segments of MSP1D1.
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E1D1 variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the acccompanying figure) of the original MSP1 sequence (which is known separately as MSP1D1), and also repeats the Helix 4 sequence of the original MSP1 sequence between the parent Helix 4 and Helix 5 segments of MSP1D1.
Application
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Legal Information
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Protocols
Nanodisc technology aids membrane protein solubilization, overcoming associated challenges in diverse protein classes.
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