MAK299
NNMT Inhibitor Screening Assay Kit
sufficient for 100 fluorometric tests
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.84
Recommended Products
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; obesity; cancer; neurological disorders
storage temp.
−70°C
General description
N′-Nicotinamide Methyltransferase (NNMT) (E.C. 2.1.1.1) catalyzes the N-methylation of nicotinamide, pyridines, and other analogues using S-adenosyl methionine (SAM) as the donor resulting in the production of 1-methylnicotinamide (MNA). NNMT plays a significant role in the regulation of metabolic pathways and is expressed at markedly high levels in several kinds of cancers, neurodegenerative diseases, obesity and diabetes, indicating it is a potential molecular target for therapy.
Suitability
Suitable for screening/studying/characterizing potential inhibitors of N′-Nicotinamide Methyltransferase (NNMT).
Principle
This NNMT inhibitor screening kit utilizes SAM as the methyl group donor and nicotinamide as the substrate. NNMT methylates nicotinamide generating S-adenosylhomocysteine (SAH) and 1-methylnicotinamide. The SAH is hydrolyzed by SAH hydrolase to form homocysteine, the free thiol group of which is detected using the Thiol Detecting Probe, generating an enhanced fluorescence signal (λex = 392 nm/ λem = 482 nm). In the presence of an NNMT inhibitor, the enzymatic activity is inhibited resulting in decreased fluorescence. This assay kit is a simple, sensitive, and rapid tool to screen potential inhibitors of NNMT.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Liq. 3 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
3 - Flammable liquids
Flash Point(F)
84.2 °F
Flash Point(C)
29 °C
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