MAK262
Sphingomyelin Quantification Colorimetric Assay Kit
sufficient for 100 colorimetric tests
Sign Into View Organizational & Contract Pricing
Select a Size
1 KIT
$737.00
All Photos(1)
Select a Size
Change View
1 KIT
$737.00
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
relevant disease(s)
neurological disorders
storage temp.
−20°C
General description
Sphingomyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomyelin is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity. Increased levels of sphingomyelin is observed in hypercholesterolemia, which is characterized by the accumulation of sphingomyelin in arteriosclerotic lesions resulting in ischemic heart disease.[1]
Suitability
Suitable for quantifying sphingomyelin in serum, plasma, and other biological fluids, and tissue and cell culture samples.
Principle
The Sphingomyelin Quantification Assay Kit is a simple and sensitive method for quantifying sphingomyelin. The assay is based on the hydrolysis of sphingomyelin into ceramide and phosphocholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphocholine to choline producing a reaction that generates a colorimetric signal (A570). The assay is sensitive to 1 M of sphingomyelin in a variety of samples.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Absorption and lipoprotein transport of sphingomyelin.
Nilsson A and Duan R D
Journal of Lipid Research, 47(1), 154-171 (2006)
Jie Xiong et al.
International journal of molecular medicine, 46(3), 1003-1012 (2020-06-26)
Alcoholic liver disease greatly affects human health. Previous studies have identified that microRNAs (miRNAs) are associated with the pathogenesis of alcoholic liver fibrosis (ALF). Therefore, the present study explored the regulatory mechanism of miR‑148a‑3p in ALF. An ALF model was established in
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
