As long as the tissue is frozen in liquid nitrogen and stored at -80 °C, it should be fine. Since ascorbate is easiliy vulnerable to oxidation, there could be some differences between the assayed values in fresh and frozen samples.
MAK075
Ascorbic Acid Assay Kit II
sufficient for 100 colorimetric tests
Synonym(s):
FRASC Assay, Ferric Reducing/Antioxidant and Ascorbic Acid
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
General description
The new Ascorbic Acid Assay Kit, MAK505, is now available! Ascorbic Acid, also known as Vitamin C, is a six-carbon lactone produced by plants and some animal species but not by humans and other primates. Ascorbic acid functions as an enzymatic cofactor for multiple enzymes, serving as an electron donor for monooxygenases and dioxygenases. Ascorbic acid also functions as a powerful antioxidant, particularly in regards to reactive oxygen species.
Application
Ascorbic Acid Assay Kit II has been used to determine ascorbic acid concentration in vitreous sample from the eye.[1]
Suitability
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
Principle
In this assay, ascorbic acid concentration is determined using the Ferric Reducing/Antioxidant and Ascorbic Acid (FRASC) assay. Fe3+ is reduced to Fe2+ by antioxidants present in the sample, which results in a colorimetric (593 nm) product. The addition of ascorbate oxidase to parallel samples oxidizes any ascorbic acid present allowing for the measurement of the ascorbic acid concentration.
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Description
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Storage Class Code
10 - Combustible liquids
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Can product MAK075, Ascorbic Acid Assay kit, be used with tissue samples which have been frozen overnight?
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