I4753

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

• Synonym(s): β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase
• CAS Number: 9001-57-4
• Enzyme Commission number: 3.2.1.26 (BRENDA, IUBMB)
• EC Number: 232-615-7
• MDL number: MFCD00131410
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Grade X
• form: solid
• specific activity: ≥300 units/mg solid
• mol wt: ~260 kDa
• foreign activity: α-galactosidase (melibiase), essentially free
• storage temp.: −20°C

Description

General description

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

Application

Invertase from Candida utilis has been used:

• as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
• to determine the sucrose content in ethanolic extract of leaf tissues
• to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Biochem/physiol Actions

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

Physical properties

Michaelis constant : 1.5x10 ^-2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70o^C
pH Stability : pH 4.0 - 6.0 (50o^C, 10min)
Thermal stability : below 60o^C (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6⁾

Unit Definition

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

Preparation Note

Chromatographically purified

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves