GE27-0361-01

Ready-to-go^™ T4 DNA Ligase

Cytiva 27-0361-01, sufficient for 50 reactions

• Synonym(s): T4 DNA ligase enzyme
• UNSPSC Code: 12352204
• NACRES: NA.53

Properties

• usage: sufficient for 50 reactions
• manufacturer/tradename: Cytiva 27-0361-01

Description

General description

Ready-To-Go^™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.

Application

Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.

Features and Benefits

• For covalently-linking DNA fragments in as little as 30 min.
• Single-dose, ambient-temperature-stable reaction mixes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Ready-To-Go is a trademark of Cytiva

Safety Information

• Storage Class Code: 13 - Non Combustible Solids