Skip to Content
MilliporeSigma
All Photos(2)

Documents

GE17-0613-10

Sephacryl® S-500 HR

Cytiva 17-0613-10, pack of 150 mL

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

packaging

pack of 150 mL

manufacturer/tradename

Cytiva 17-0613-10

matrix

allyl dextran and N, N’-methylenebisacrylamide

particle size

25-75 μm

avg. part. size

50 μm

cleaning in place

2-13

working range

3-11

suitability

suitable for bioprocess medium

General description

Sephacryl® High Resolution gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Application

Sephacryl® High Resolution chromatography media is a highly versatile gel filtration media that offers a wide range of fractionation capabilities. The matrix of Sephacryl® High Resolution media is a cross-linked copolymer of allyl Dextran and N,N′-methylene bisacrylamide. This crosslinking gives good rigidity and chemical stability. The narrow particle size distribution, together with steep selectivity curves, results in good preparative characteristics with maintained high resolution. The hydrophilic nature of the media minimizes nonspecific adsorption and maximizes recovery. The excellent resolution and flow characteristics, long-term physical and chemical stability, and ease of handling make Sephacryl® HR the medium of choice for routine purification.

Sephacryl® High Resolution is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As a BioProcess medium, Sephacryl® High Resolution, meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Cost-efficient gel filtration media providing high resolution with fast and easy operation.
  • Five different fractionation ranges available in Sephacryl® HR family allows for reproducible separations over a wide molecular weight range
  • Sephacryl® S-500HR High Resolution medium is recommended to separate polysaccharides, macromolecules with extended structures and even small particles such as plasmids (fractionation range Dextrans 4 × 10exp4–2 × 107)
  • BioProcess medium supported for industrial applications and well-established in approved processes.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sephacryl is a registered trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Performing an Isolation of Recombinant Protein Complexes

This page provides information about performing an isolation of recombinant protein complexes with different pull-down assays with products from Cytiva.

Protocols

Performing an Isolation of Native Complexes

This page provides information about performing an isolation of natural multi-protein complexes for structural and functional studies using classical biochemical methods and products from Cytiva.

Column Packing and Preparation for Size Exclusion Chromatography

Column Packing and Preparation for Size Exclusion Chromatography

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service