G5671
Anti-Galectin-8 antibody, Mouse monoclonal
~1.0 mg/mL, clone VA-11.25, purified from hybridoma cell culture
Synonym(s):
ANTIGal-8, ANTILGALS8, ANTILectin, galectoside-binding, soluble 8, ANTIPCTA1, ANTIProstate carcinoma tumor antigen 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
VA-11.25, monoclonal
form
buffered aqueous solution
mol wt
antigen ~34 kDa
species reactivity
mouse, rat, human
concentration
~1.0 mg/mL
technique(s)
immunocytochemistry: suitable
indirect ELISA: suitable
western blot: 1-2 μg/mL using rat galectin 8 recombinant protein
isotype
IgM
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LGALS8(3964)
mouse ... Lgals8(56048)
rat ... Lgals8(116641)
General description
Galectins specifically bind to β-galactosides. 15 mammalian galectins have been identified so far. Galectin 8 contains two CRDs connected by a short linker peptide.
Monoclonal Anti-Galectin 8 (mouse IgM isotype) is derived from the hybridoma VA-11.25 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant rat galectin 8.Galectins belong to a family of carbohydrate-binding proteins. Within this family of proteins, galectin 8 is unique, as it exists in many forms encoded by the same gene.
Specificity
The antibody has low cross-reactivity with galectin 3 and galectin 9.
Immunogen
recombinant rat galectin 8.
Application
Monoclonal Anti-galectin-8 antibody produced in mouse has been used for:
- immunoblotting
- immunocytochemistry
- enzyme linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Galectins belong to a family of carbohydrate-binding proteins. These proteins share similarities in the carbohydrate recognition domain (CRD) and in their specificity for N-Acetyllactosamine-enriched glycoconjugates. The binding of galectins to saccharides modulates cell proliferation, cell death and cell migration, which are involved in cancer initiation and progression. Galectin 8 is a secreted protein that requires both of its CRDs to be active to modulate cell adhesion. It forms a complex with integrins and triggers integrin-mediated signaling cascades. On the contrary, excessive amounts of this protein negatively regulate cell adhesion by interacting with integrins. These signals assist in identifying regions available for cell adhesion and migration. It may be involved in neoplastic transformation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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G A Rabinovich
Cell death and differentiation, 6(8), 711-721 (1999-09-01)
Galectins constitute a family of evolutionarily conserved animal lectins, which are defined by their affinity for poly-N-acetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain. During the past decade, attempts to dissect the functional role for galectins in vivo
Human galectin-8 isoforms and cancer
Bidon WN and Le Pennec JP
Glycoconjugate Journal, 19(7-9), 557-563 (2002)
Y Levy et al.
The Journal of biological chemistry, 276(33), 31285-31295 (2001-05-24)
The interaction of cells with the extracellular matrix regulates cell adhesion and motility. Here we demonstrate that different cell types adhere and spread when cultured in serum-free medium on immobilized galectin-8, a mammalian beta-galactoside-binding protein. At maximal doses, galectin-8 is
Yehiel Zick et al.
Glycoconjugate journal, 19(7-9), 517-526 (2004-02-06)
Galectin-8 belongs to the family of tandem-repeat type galectins. It consists as several isoforms, each made of two domains of approximately 140 amino-acids, both having a carbohydrate recognition domain (CRD). These domains are joined by a 'link peptide' of variable
Hitomi Yamamoto et al.
Journal of biochemistry, 143(3), 311-324 (2007-11-21)
We previously showed that tandem-repeat type galectin-8, which has two covalently linked carbohydrate recognition domains (CRDs), induces neutrophil-adhesion through binding to integrin alphaM. Here, we analysed the function of galectin-8 in Jurkat T-cells. Galectin-8, as well as tandem-repeat galectin-9, and
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