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E9788

Sigma-Aldrich

Cytochrome P450 1A2 human

recombinant, expressed in Saccharomyces cerevisiae

Synonym(s):

Cytochrome P450 Microsome Preparation human, Cerosomes

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About This Item

Enzyme Commission number:
1.14.14.1 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

human

Quality Level

200

recombinant

expressed in Saccharomyces cerevisiae

form

liquid

specific activity

≥0.5 units/pmol enzyme

secondary activity

≥100 units/mg protein (Reductase activity)

concentration

≥1 pmol/μL (P450)
≥10 mg/mL protein

UniProt accession no.

P05177

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP1A2(1544)

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General description

Microsome contents of CYP1A2 human and yeast CYP-reductase expressed in Saccharomyces cerevisiae.

Unit Definition

P450 Activity: One unit will oxidize 1 picomole of ethoxyresorufin per minute at pH 7.4 at 28 °C.
Reductase Activity: One unit will reduce 1 nanomole of cytochrome C per minute in the presence of NADPH at pH 7.4 at 28 °C.

Physical form

50 mM Tris (pH 7.4), 1 mM EDTA, 20% glycerol

Other Notes

For more more information visit our drug metabolism resources for Cytochrome P450 microsome preparations.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Gowtham Sathyanarayanan et al.
Micromachines, 9(12) (2018-12-14)
This work describes the interfacing of electrowetting-on-dielectric based digital microfluidic (DMF) sample preparation devices with ambient mass spectrometry (MS) via desorption atmospheric pressure photoionization (DAPPI). The DMF droplet manipulation technique was adopted to facilitate drug distribution and metabolism assays in
Haining Zhou et al.
Chemical research in toxicology, 33(7), 1960-1968 (2020-06-03)
Fraxinellone, a furanoid, is one of the bioactive and potentially hepatotoxic constituents from Dictamnus dasycarpus Turcz, which is extensively spread throughout Asian countries. This herb was reported to cause liver injury in clinical application. However, the mechanism behind is still
Miyabi Ito et al.
Drug metabolism and pharmacokinetics, 30(3), 247-252 (2015-05-30)
Genetic variations in cytochrome P450 1A2 (CYP1A2) are associated with interindividual variability in the metabolism and efficacy of many medications. Twenty CYP1A2 variants harboring amino acid substitutions were analyzed for functional changes in enzymatic activity. Recombinant CYP1A2 variant proteins were

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