E1894

EPPS

BioXtra, ≥99.5% (titration)

• Synonym(s): Triethanolamine hydrochloric acid salt, 4-(2-Hydroxyethyl)-1-piperazinepropanesulfonic acid, 4-(2-Hydroxyethyl)piperazine-1-propanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(3-propanesulfonic acid), HEPPS
• Empirical Formula (Hill Notation): C₉H₂₀N₂O₄S
• CAS Number: 16052-06-5
• Molecular Weight: 252.33
• Beilstein: 3957385
• EC Number: 240-198-8
• MDL number: MFCD00006160
• UNSPSC Code: 12161700
• PubChem Substance ID: 24894443
• NACRES: NA.25

Properties

• product line: BioXtra
• Quality Level: 400
• Assay: ≥99.5% (titration)
• form: powder
• impurities: ≤0.005% Phosphorus (P); ≤0.1% Insoluble matter
• ign. residue: ≤0.1%
• pH: 5.0-7.0 (20 °C, 1 M in H₂O)
• useful pH range: 7.3-8.7
• pKa: 8
• mp: 237-239 °C (lit.)
• solubility: H₂O: 1 M at 20 °C, clear, colorless
• anion traces: bromide (Br^-): ≤0.1%
• cation traces: Al: ≤0.0005%; Ca: ≤0.002%; Cu: ≤0.0005%; Fe: ≤0.0005%; K: ≤0.005%; Mg: ≤0.0005%; NH₄⁺: ≤0.1%; Na: ≤0.005%; Pb: ≤0.001%; Zn: ≤0.0005%
• absorption: ≤0.1 at 260 in H₂O at 1 M; ≤0.1 at 280 in H₂O at 1 M
• application(s): diagnostic assay manufacturing
• SMILES string: OCCN1CCN(CCCS(O)(=O)=O)CC1
• InChI: 1S/C9H20N2O4S/c12-8-7-11-5-3-10(4-6-11)2-1-9-16(13,14)15/h12H,1-9H2,(H,13,14,15)
• InChI key: OWXMKDGYPWMGEB-UHFFFAOYSA-N

Description

General description

EPPS, also known as 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid, is a zwitterionic buffering agent that belongs to the group of good buffers. It plays a vital role in maintaining a stable pH in solutions, which is critical for various biological and biochemical processes. EPPS buffer finds extensive application in biological research and cell culture experiments.

Application

EPPS buffer has been used as a component of buffer solution for methylglyoxal (MG) treatment in vitro to form advanced glycation end products (AGEs) to crosslink collagen fibrils. It has also been used in the preparation of fixation buffer solution for light microscopy.

Features and Benefits

• The BioXtra product line ensures T15 high-quality products for consistent results.
• A high assay of ≥99.5% guarantees the purity of the product.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves