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DFF100

Sigma-Aldrich

DEAE–Sepharose™

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Synonym(s):

Diethylaminoethyl–Sepharose™

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About This Item

CAS Number:
57407-08-6
MDL number:
MFCD00146630
UNSPSC Code:
47101511
NACRES:
NA.56

Quality Level

200

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

bead size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

pH

2—12

capacity

110-160 μeq/mL binding capacity(gel volume)

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General description

DFF100-500Ml′s update product number is GE17-0709-01

Application

DEAE-Sepharose has been used in:
  • anion exchange chromatography
  • to screen polyisoprenyl phosphate phosphatase activity
  • to purify isoinhibitors
  • for the purification of human immunodeficiency virus (HIV)
  • glycoprotein envelope (gp140 env)

DEAE-Sepharose™ is used in affinity chromatography, protein chromatography and ion exchange chromatography. DEAE-Sepharose™ has been used to study pathogenesis of human disease and to develop a new assay for detecting the toxins of pathogenic strains of Clostridium difficile.

Biochem/physiol Actions

Diethylaminoethyl-sepharose (DEAE-sepharose) is a strong anion exchange column, where DEAE covalently binds to sepharose.

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Product No.
Description
Pricing

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rapid high-level production of functional HIV broadly neutralizing monoclonal antibodies in transient plant expression systems
Rosenberg Y, et al.
Testing, 8(3), e58724-e58724 (2013)
Effective Purification Procedure of <I>Aspergillus oryzae</I> α−Amylase from Solid State Fermentation Cultures Including Concanavalin-A Sepharose
Terebiznik, M.R., et al.
Journal of Food Biochemistry, 19(5), 341-354 (1995)
Growth cone collapse and neurite retraction from cultured <I>Helisoma</I> neurons in response to antibody Fab fragments against an extracellular matrix protein.
Miller, J.D., et al.
Dev. Brain Res., 79(2), 203-218 (1994)
Valérie Molinier-Frenkel et al.
Journal of virology, 76(1), 127-135 (2001-12-12)
The capacity of recombinant adenoviruses (rAd) to induce immunization against their transgene products has been well documented. In the present study, we evaluated the vaccinal adjuvant role of rAd independently of its vector function. BALB/c mice received one subcutaneous injection
G L Yewey et al.
Biochemical and biophysical research communications, 148(3), 1520-1526 (1987-11-13)
Bovine heart cytochrome c oxidase, depleted of polypeptide subunits by alkaline detergent treatment, was characterized with respect to metal content, optical spectral properties, and oxidase activity. Treatment with 1.0% Triton X-100 at pH 9.5 followed by anion-exchange chromatography caused removal
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