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D1145

Sigma-Aldrich

DMEM - high glucose

Dulbecco’s Modified Eagle’s Medium - high glucose

With sodium bicarbonate, without ʟ-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell cutlure

Synonym(s):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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500 ML
$44.80
6 X 500 ML
$234.00

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500 ML
$44.80
6 X 500 ML
$234.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.71

$44.80

In StockDetails

Request a Bulk Order

Product Name

Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and sodium bicarbonate, without L-glutamine, sodium pyruvate, and phenol red, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: no
sodium pyruvate: no
glucose: high
phenol red: no
NaHCO3: yes
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Reconstitution

Supplement with 0.584 g/L L-glutamine.

also commonly purchased with this product

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Description
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supplement

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBM0067
RNBL6320
RNBL4449
RNBL1001
RNBK9782

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Ştefan Ţălu et al.
Molecular vision, 24, 902-912 (2019-02-05)
This study aimed to quantify the three-dimensional micromorphology of the surface of the human lens capsule as a function of age. Imaging experiments were conducted on whole human lenses received from eight human cadavers (donor age range: 30-88 years). Imaging
Tracy A O'Mara et al.
Nature communications, 9(1), 3166-3166 (2018-08-11)
Endometrial cancer is the most commonly diagnosed cancer of the female reproductive tract in developed countries. Through genome-wide association studies (GWAS), we have previously identified eight risk loci for endometrial cancer. Here, we present an expanded meta-analysis of 12,906 endometrial
Jikai Zhao et al.
The Journal of steroid biochemistry and molecular biology, 178, 354-368 (2018-02-17)
Hippocampal synaptic plasticity is the basis of spatial memory and cognition and is strongly regulated by both testicular androgens (testosterone, T) and hippocampal estrogens (17β-estradiol, E2) converted from T by aromatase, which is inhibited by letrozole (LET), but the contribution
Sina Naddaf Dezfuli et al.
Materials science & engineering. C, Materials for biological applications, 79, 647-660 (2017-06-21)
The present research was aimed at developing magnesium-matrix composites that could allow effective control over their physiochemical and mechanical responses when in contact with physiological solutions. A biodegradable, bioactive ceramic - bredigite was chosen as the reinforcing phase in the
Shinsuke Matsuzaki et al.
PloS one, 10(4), e0123578-e0123578 (2015-05-01)
Recently, accumulating reports have suggested the importance of endoplasmic reticulum (ER) stress signaling in the differentiation of several tissues and cells, including myoblasts and osteoblasts. Secretory cells are easily subjected to ER stress during maturation of their secreted proteins. Skin
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Questions

  1. Can this media be used to culture COS-7 cell?

    1 answer

    1. Yes, Product D1145 has been cited by the following publication to culture COS-7 cells.

      Nat Commun. 2021 Jul 5;12(1):4132.
      A synthetic circuit for buffering gene dosage variation between individual mammalian cells
      https://pubmed.ncbi.nlm.nih.gov/34226556/

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Reviews

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  1. Charlottesville VA
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Suitability of medium for immunofluorecence applic

    Convenient formulation that allows cells grown to undergo processing for immunofluorescence imaging.

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