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Key Documents

LB205

98062329, human blood, Lymphoblastoid

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About This Item

UNSPSC Code:
41106514

product name

LB205, 98062329

biological source

human blood

growth mode

Suspension

karyotype

Not specified

morphology

Lymphoblastoid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human lymphocytes, Xeroderma pigmentosum variant, EBV-transformed

Cell Line Description

Derived from mononuclear cells from the peripheral blood of a 62 year old male. The patient was diagnosed with Xeroderma pigmentosum variant, illustrating that the disease is heterogeneous. T cells from the original sample have been used for UV irradiation studies and hprt mutant frequency. The cell line has been established by transformation with EBV. Fibroblasts from the same individual are designated XP11 BR.

Application

DNA repair deficiency studies, hprt mutation analysis

Culture Medium

RPMI 1640 + 4mM Glutamine + 200 μg/ml Sodium Pyruvate (NaPy) + 15% Heat Inactivated (HI-FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml dilute every 1-2 days to 3 x 100,000 cells/ml 5%

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Certificates of Analysis (COA)

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