LL47 (MaDo)

90102538, human lung, Fibroblast-like

• UNSPSC Code: 41106514

Properties

• product name: LL47 (MaDo), 90102538
• biological source: human lung
• growth mode: Adherent
• karyotype: 2n = 46; diploid
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human diploid lung

Cell Line Description

Derived from normal lung tissue obtained from a 16 year old male with osteogenic sarcoma. Ethnicity: Black.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,12
D13S317: 9,11
D16S539: 11
D5S818: 12,13
D7S820: 8,10
THO1: 7,9.3
TPOX: 9,10
vWA: 14,16

Culture Medium

Coon′s modified Ham′s F12 + Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. A further 15 population doublings expected.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.