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product name
Nthy-ts1, 90011610
biological source
human thyroid
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human thyroid follicular mutant epithelial
Cell Line Description
Normal primary cultures of human thyroid follicular cells were transfected with a plasmid containing the SV40 early region large T encoding gene from mutant tsA58. Tight temperature dependence for growth is observed: Nthy-ts1 cells grow at 33°C, but not at the restrictive temperature of 40.5°C. This SV40 mutant allows the study of oncogene action free of any interfering effects of SV40 large T antigen.
Application
Transfection, mutation and virus studies: SV40
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33°C.
Other Notes
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