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biological source
Mouse x Mouse Hybridoma
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0/1
Reactivity
Anti-CD25, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse NS0/1 x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against the rat IL-2 receptor found on T cell blasts and thymic dendritic cells. The OX-39 antigen has a molecular weight of 45-50kDa.
Immunogen
T blasts from a mixed lymphocyte reaction between purified CD4+ T cells and irradiated spleens
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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