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form
liquid
packaging
pkg of 250 ml
technique(s)
cell culture | mammalian: suitable
shipped in
wet ice
General description
Cancer Cell Line Medium XF
Application
Cancer Cell Line Medium XF is a cell culture medium developed for the standardized in vitro cultivation of established human cancer cell lines. Its serum-free and xeno-free formulation provides a culture environment devoid of all stimuli originating from non-defined materials. Thus, it has no ill-defined components such as fetal calf serum, extracts or hydrolysates and exhibits very low lot-to-lot variability. The medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete medium.
Quality
All lots of the PromoCell Cancer Cell Line Medium XF are subjected to comprehensive quality control tests using a human cancer cell line. Each lot of PromoCell Cancer Cell Line Medium XF is tested for the ability to support proliferation and serial passage of MCF7 breast carcinoma cells on fibronectincoated dishes. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Storage and Stability
Store the Basal Medium at 2 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 2 to 8°C. For use, prewarm only an aliquot of the complete medium and keep the remaining medium refrigerated at 2 to 8°C.
Subculture Routine
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zhikai Wang et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(4) (2022-01-21)
Cancer immunotherapy frequently fails because most carcinomas have few T cells, suggesting that cancers can suppress T cell infiltration. Here, we show that cancer cells of human pancreatic ductal adenocarcinoma (PDA), colorectal cancer, and breast cancer are coated with transglutaminase-2
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Defined culture conditions. Consistent growth of human cancer cell lines in PromoCell’s serum-free/xeno-free Cancer Cell Line Media-XF. Explore over 350 PromoCell products on Sigma.com
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