94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Synonym(s): Urate oxidase
• CAS Number: 9002-12-4
• Enzyme Commission number: 1.7.3.3 (BRENDA, IUBMB)
• MDL number: MFCD00082126
• UNSPSC Code: 12352204
• PubChem Substance ID: 57653596
• NACRES: NA.54

Properties

• biological source: Bacillus sp. (B. fastidiosus)
• form: powder
• quality: lyophilized
• specific activity: ~9 U/mg
• storage condition: dry at room temperature
• concentration: 90—100%
• color: white to faint beige
• solubility: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• application(s): detection
• storage temp.: −20°C
• SMILES string: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChI key: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Description

General description

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Application

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Biochem/physiol Actions

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Unit Definition

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Other Notes

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H319,H334,H360FD
• Precautionary Statements: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves