Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

View All Documentation

93482

Sigma-Aldrich

Phenylmethanesulfonyl fluoride solution

~0.1 M in ethanol (T)

Synonym(s):

Benzylsulfonyl fluoride, PMSF

Sign Into View Organizational & Contract Pricing

Select a Size

50 ML
$172.00
250 ML
$713.00

$172.00

In StockDetails

All Photos(1)

Select a Size

Change View
50 ML
$172.00
250 ML
$713.00

About This Item

CAS Number:
329-98-6
Beilstein:
2088311
MDL number:
MFCD00007424
UNSPSC Code:
12352202
PubChem Substance ID:
57653563
NACRES:
NA.77

$172.00

In StockDetails

biological source

microbial
synthetic

Quality Level

200

form

liquid

concentration

~0.1 M in ethanol (T)

density

0.797 g/mL at 20 °C

storage temp.

2-8°C

SMILES string

FS(=O)(=O)Cc1ccccc1

InChI

1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2

InChI key

YBYRMVIVWMBXKQ-UHFFFAOYSA-N

Application

Phenylmethanesulfonyl fluoride solution has been used as a component in the cell lysate buffer in breast adenocarcinoma cell line[1], fibrosarcoma cell line[2] and D5 hindbrain and spinal cord neural progenitors.[3]

Biochem/physiol Actions

Administration of PMSF produces analgesia unrelated to its anticholinesterase effect, and prolongs the analagesic effect of centrally administered β-endorphin.[4]
Phenylmethanesulfonyl fluoride (PMSF) is a serine protease inhibitor. It binds covalently to active site residue of proteases and prevents proteolytic activity. PMSF is used in cell lysate buffers during protein purification to prevent target protein degradation.[5] PMSF effectively inhibits the naloxone-precipitated withdrawal jumping.[6]

Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225,H315,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCM0399
BCCL2616
BCCL1411
BCCK3515
BCCH5056

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

SREBP1 drives KRT80-dependent cytoskeletal changes and invasive behavior in endocrine resistant ERalpha breast cancer
Magnani L, et al.
bioRxiv, 380634-380634 (2018)
Nervous system regionalization entails axial allocation before neural differentiation
Metzis V, et al.
Cell, 175(4), 1105-1118 (2018)
DNA-encoded library-derived DDR1 inhibitor prevents fibrosis and renal function loss in a genetic mouse model of Alport syndrome
Richter H, et al.
ACS Chemical Biology (2018)
Phenylmethanesulfonyl fluoride, a serine protease inhibitor, suppresses naloxone-precipitated withdrawal jumping in morphine-dependent mice
Nemoto W, et al.
Neuropeptides, 47(3), 187-191 (2013)
Jih-Jung Chen et al.
Journal of natural products, 71(1), 71-75 (2008-01-01)
Two new benzoic acid derivatives, (E)-3-acetyl-6-(3,7-dimethylocta-2,6-dienyloxy)-2,4-dihydroxybenzoic acid (1) and (E)-3-acetyl-4-(3,7-dimethylocta-2,6-dienyloxy)-2,6-dihydroxybenzoic acid (2), and three new acetophenones, (E)-1-(5-(3,7-dimethylocta-2,6-dienyloxy)-7-hydroxy-2,2-dimethyl-2 H-chromen-8-yl)ethanone (3), (E)-1-(5-(3,7-dimethylocta-2,6-dienyloxy)-7-hydroxy-2-methyl-2-(4-methylpent-3-enyl)-2 H-chromen-8-yl)ethanone (4), and (R,E)-1-(5-(3,7-dimethylocta-2,6-dienyloxy)-3,7-dihydroxy-2,2-dimethylchroman-8-yl)ethanone (5), have been isolated from the fruits of Melicope semecarpifolia, together with eight known compounds. The
View All Related Papers

Questions

1–4 of 4 Questions  

  1. Is this product 93482 RNase-free?

    1 answer

    1. This product is not tested for RNase activity. Currently, an RNAse tested PMSF product is not available.

      Helpful?

  2. How can I further dilute Product 93482, Phenylmethanesulfonyl fluoride solution?

    1 answer

    1. We would recommend that you use an anhydrous ethanol to further dilute this solution, since PMSF is very unstable in water.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What can Product 93482, Phenylmethanesulfonyl fluoride solution, be used for?

    1 answer

    1. PMSF is a serine protease inhibitor, inhibiting the activity of enzymes such as thrombin, trypsin, papain, chymotrypsin and proteinase K. It also inhibits mammalian acetylcholinesterases.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service