Antonie van Leeuwenhoek, 80(3-4), 225-236 (2002-02-06)
Flocculation of yeasts is a cell-cell aggregation phenomenon which is driven by interactions between cell wall lectins and cell wall heteropolysaccharides. In Sabouraud medium, Kluyveromyces bulgaricus was highly flocculent. Incubation of flocculent K. bulgaricus cells with EDTA or Hecameg led
Biochimica et biophysica acta, 1193(2), 301-306 (1994-08-03)
A number of properties and effects of the novel non-ionic detergent Hecameg (6-O-(N-heptylcarbamoyl)-methyl-alpha-D-glucopyranoside) have been examined in view of its possible biochemical applications. In particular, its critical micellar concentration has been measured, and its effects on pure lipid membranes, soluble
Applied microbiology and biotechnology, 49(1), 16-23 (1998-03-06)
The extraction of a lectinic factor involved in yeast flocculation, from two Kluyveromyces lactis strains (a flocculent K. lactis 5c and a non-flocculent K. lactis 5a strain) was performed using EDTA and two surfactants, Hecameg and HTAC. The properties of
The lectin-like theory suggest that yeast flocculation is mediated by an aggregating lectinic factor. In this study we isolated an agglutinating factor, which corresponds to lectin, from whole cells by treating the flocculent wild-type Saccharomyces cerevisiae NCYC 625 strain and
Surfactants carrying either a hydrocarbon or a fluorocarbon alkyl chain have been synthesized. The polar head was either tris(hydroxymethyl)acrylamidomethane (THAM), telomerized THAM, or a glycosylated THAM moiety. The aqueous solubility of some of these molecules was increased by oxidizing to
This page shows how to solubilize membrane proteins with products from Cytiva.
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