57180

Indo 1

for fluorescence, ≥80.0% (HPLC)

• Synonym(s): 4-(6-Carboxy-2-indolyl)-4′-methyl-2,2′-(ethylenedioxy)dianiline-N,N,N′,N′-tetraacetic acid pentapotassium salt
• Empirical Formula (Hill Notation): C₃₂H₂₆K₅N₃O₁₂
• CAS Number: 132319-56-3
• Molecular Weight: 840.05
• MDL number: MFCD00077797
• UNSPSC Code: 12352200
• PubChem Substance ID: 329758276
• NACRES: NA.32

Properties

• grade: for fluorescence
• Assay: ≥80.0% (HPLC)
• form: solid
• fluorescence: λ_ex 331 nm; λ_em 398 nm in 0.1 M Tris pH 8.0, 10 mM Ca²⁺; λ_ex 349 nm; λ_em 482 nm (100mM KCI)
• suitability: in accordance for fluorescence
• storage temp.: −20°C
• SMILES string: [K+].[K+].[K+].[K+].[K+].Cc1ccc(N(CC([O-])=O)CC([O-])=O)c(OCCOc2cc(ccc2N(CC([O-])=O)CC([O-])=O)-c3cc4ccc(cc4[nH]3)C([O-])=O)c1
• InChI: 1S/C32H31N3O12.5K/c1-18-2-6-24(34(14-28(36)37)15-29(38)39)26(10-18)46-8-9-47-27-13-20(5-7-25(27)35(16-30(40)41)17-31(42)43)22-11-19-3-4-21(32(44)45)12-23(19)33-22;;;;;/h2-7,10-13,33H,8-9,14-17H2,1H3,(H,36,37)(H,38,39)(H,40,41)(H,42,43)(H,44,45);;;;;/q;5*+1/p-5
• InChI key: VGZPRMSXVNDMAN-UHFFFAOYSA-I

Description

Application

Indo 1, a fluorescent calcium chelator and indicator, is used to monitor cytoplasmic Ca2+ transients induced by various physiological and mechanical processes.

Other Notes

Fluorescent calcium chelator and indicator with an unusually large shift in fluorescence emission from 480 nm to 400 nm on binding of calcium. Useful for intracellular application

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves