349M-9

ZAP-70 (2F3.2) Mouse Monoclonal Antibody

• UNSPSC Code: 12352202
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 2F3.2, monoclonal
• description: For In Vitro Diagnostic Use in Select Regions (See Chart)
• form: buffered aqueous solution
• species reactivity: human
• packaging: vial of 0.1 mL concentrate (349M-94); vial of 0.5 mL concentrate (349M-95); bottle of 1.0 mL predilute (349M-97); vial of 1.0 mL concentrate (349M-96); bottle of 7.0 mL predilute (349M-98)
• manufacturer/tradename: Cell Marque^™
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500
• isotype: IgG2a
• control: chronic lymphocytic leukemia
• shipped in: wet ice
• storage temp.: 2-8°C
• visualization: cytoplasmic
• Gene Information: human ... ZAP70(7535)

Description

General description

Zeta-associated protein-70 (ZAP-70), a member of the Syk family of tyrosine kinases, has an important role in T-cell receptor signaling, natural killer cell activation, and early B-cell development. ZAP-70 protein is not expressed in most normal mature B-cells, but is expressed in various B- and T-cell lymphomas. Attempts to implement ZAP-70 analysis in clinical flow cytometry laboratories have been fraught with difficulty; this approach is technically challenging and results often have been unreliable. Detection of ZAP-70 protein by immunohistochemistry using tissue sections of chronic lymphocytic leukemia/small lymphocyctic lymphoma (CLL/SLL) is an easy and inexpensive technique. The clinical course of CLL/SLL is extremely variable: in some patients it is aggressive and requires chemotherapy within a relatively short time of diagnosis; in others it is indolent and asymptomatic and may not require treatment for many years. It has recently been demonstrated that the mutational status of the immunoglobulin heavy-chain variable-region (IgVH) genes identifies 2 distinct groups of CLL/SLL: the patients with unmutated IgVH have an unfavorable clinical course, whereas those with mutated IgVH experience a favorable course. The reasons for the differences in the clinical course of CLL patients with mutated and unmutated IgVH genes are not clear. ZAP-70 expression is the best discriminator of mutated and unmutated IgVH genes, ZAP-70 positive (>20% tumor cells) being Ig-unmutated and ZAP-70 negative being Ig-mutated. Wiestner et al. demonstrated that ZAP-70 expression levels assessed by means of Western blotting, real-time RT-PCR and immunohistochemistry are closely correlated with IgVH mutational status.

Quality

IVD

IVD

IVD

NA

Linkage

ZAP-70 Positive Control Slides, Product No. 349S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Preparation Note

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Other Notes

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Legal Information

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable