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21115

Sigma-Aldrich

Calcium chloride solution

BioUltra, for molecular biology, ~1 M in H2O

Synonym(s):

CaCl2, Calcium chloride hydrate, Calcium dichloride

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About This Item

Empirical Formula (Hill Notation):
CaCl2
CAS Number:
Molecular Weight:
110.98
MDL number:
UNSPSC Code:
12352302
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

refractive index

n20/D 1.358

pH

4.0-6.0 (25 °C)

density

1.086 g/mL at 20 °C

cation traces

Ba: ≤1 mg/kg
Bi: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤1 mg/kg
Mg: ≤5 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Na: ≤20 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤20 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.02

SMILES string

Cl[Ca]Cl

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

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Application

Calcium chloride solution has been used in hippocampal slice culture preparation and HEPES-buffered tyrode′s solution.

Other Notes

In electric-field induced DNA transfection - increased by millimolar concentrations of calcium chloride.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

To measure collagenase activity, N-(3-[2-Furyl]acryloyl)-Leu-Gly-Pro-Ala is used in a continuous spectrophotometric rate determination at 345 nm. Collagenase hydrolyzes collagen peptide bonds.

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