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270733

Sigma-Aldrich

Water

Water
1 of 1 reviewers received a sample product or took part in a promotion

suitable for HPLC

Synonym(s):

Aqua, Dihydrogen oxide

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1 L
$82.10
4 L
$188.00
4 X 4 L
$571.00
20 L
$799.00

$82.10


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1 L
$82.10
4 L
$188.00
4 X 4 L
$571.00
20 L
$799.00

About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC Code:
12190000
PubChem Substance ID:
NACRES:
NA.21
technique(s):
HPLC: suitable
bp:
100 °C (lit.)
vapor pressure:
3 mmHg

$82.10


In StockDetails


Request a Bulk Order

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

form

liquid

technique(s)

HPLC: suitable

evapn. residue

<0.0003%

refractive index

n20/D 1.34 (lit.)

pH

6.0-8.0 (25 °C)

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

λ

H2O reference

UV absorption

λ: 205 nm Amax: 0.01
λ: 210 nm Amax: 0.01
λ: 250-400 nm Amax: 0.005

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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General description

Water suitable for HPLC is commonly used as a solvent for isocratic elution in both cation and anion chromatography. Additionally, it is used as a component to prepare standard stock solutions, and for evaporation under a nitrogen stream in a water bath to concentrate the sample before LC-MS-MS analysis.[1] [2]

Application

Water is used as a:
  • Solvent to prepare buffers during the cell lysis process.[3]
  • Component of the mobile phase during the HPLC–UV–diode-array detection for the separation and detection of the analytes.[4]

Preparation Note

Product filtered through a 0.2 μm filter

Other Notes

Important notice
The article number 270733-6X1L will be discontinued. Please order the single bottle 270733-1L which is physically identical with the same exact specifications.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Certificates of Analysis (COA)

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Questions

1–6 of 6 Questions  
  1. Do you know the resistivity value @ 25°C?

    1 answer
    1. The resistivity of this product has not been determined.

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  4. How is this water purified? is it distilled?

    1 answer
    1. The water purification process involves several steps:
      • The water initially passes through a carbon exchange tank to remove organic compounds.
      • It is then filtered using a 1μm PE cartridge filter to remove any remaining impurities.
      • Inorganic compounds are eliminated through deionization tanks, ensuring high purity. The resistivity of the water is checked during this stage.
      •The water undergoes sterilization using UV light to eliminate any microorganisms.
      • Finally, the water is filtered through a 0.2μm cartridge filter, and the resulting HPLC grade water is packaged in glass bottles.
      Note: This information provides an overview of the typical water purification process and may vary depending on specific applications or systems.

      Helpful?

  5. Do you know the concentration of TOC in this water?

    1 answer
    1. The concentration of TOC in this product is not determined. Product 78533, Water for TOC Analysis, may be of interest.
      Please see the link below to review this product option:
      https://www.sigmaaldrich.com/product/sial/78533

      Helpful?

  6. Is this product (HPLC grade water #270733) DNase-, RNase- and protease-free?

    1 answer
    1. The item is not tested for the presence of DNA, RNA, and protease.

      Please see the links below to review product options that include this testing:
      W4502
      https://www.sigmaaldrich.com/US/en/product/sigma/w4502
      95284
      https://www.sigmaaldrich.com/US/en/product/sigma/95284

      Note that the above options are NOT tested for suitability in HPLC.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

    • Reviews 3
    • Votes 9
    5 out of 5 stars.

    Good, pure water

    Water was high purity, HPLC grade. We didn't actually use it for HPLC but it seemed very clean and pure for our purposes.

    Helpful?

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